Inhibition of west nile virus replication by retrovirus‐delivered small interfering RNA in human neuroblastoma cells
Identifieur interne : 003345 ( Main/Exploration ); précédent : 003344; suivant : 003346Inhibition of west nile virus replication by retrovirus‐delivered small interfering RNA in human neuroblastoma cells
Auteurs : Yongbo Yang [États-Unis, République populaire de Chine] ; Chengxiang Wu [États-Unis] ; Jianguo Wu [République populaire de Chine] ; Vivek R. Nerurkar [États-Unis] ; Richard Yanagihara [États-Unis] ; Yuanan Lu [États-Unis]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 2008-05.
English descriptors
- Teeft :
- Antiviral therapy, Assay, Burns school, Cell medium, Cells transduced, Control cells, Different regions, Endogenous reference, Envelope protein, Experimental control, Exponential growth phase, Expression level, Gene, Gene expression, Genome, Grant number, Grant sponsor, Human cells, Human neuroblastoma cell line, Human neuroblastoma cells, Inhibition, Inhibitory effect, Inhibitory effects, Inhibitory impact, Internal control, Lentivirus vectors, Mammalian cells, Negative control, Neuroblastoma, Neuroblastoma cells, Nile, Other studies, Plaque, Plaque assay, Plasmid vectors, Proc natl acad, Public health sciences, Recent reports, Replication, Retroviral, Retroviral delivery, Retroviral sirna expression, Retroviral vector, Retroviral vectors, Rna, Rnai, Short hairpin, Sirna, Sirna cells, Sirna expression, Sirna sequences, Structural proteins, Target sites, Transduced, Transduced cells, Untransduced control cells, Viral, Viral proteins, Virol, Virus, Virus production, Virus replication, West nile virus, West nile virus replication, Western blot analysis.
Abstract
West Nile virus (WNV) has been responsible for the largest outbreaks of arboviral encephalitis in U.S. history. No specific drug is currently available for the effective treatment of WNV infection. To exploit RNA interference as a potential therapeutic approach, a Moloney murine leukemia virus‐based retrovirus vector was used to effectively deliver WNV‐specific small interfering RNA (siRNA) into human neuroblastoma HTB‐11 cells. Viral plaque assays demonstrated that transduced cells were significantly refractory to WNV replication, as compared to untransduced control cells (P < 0.05), which correlated with the reduced expression of target viral genes and respective viral proteins. Therefore, retrovirus‐mediated delivery of siRNA for gene silencing can be used to study the specific functions of viral genes associated with replication and may have potential therapeutic applications. J. Med. Virol. 80:930–936, 2008. © 2008 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jmv.21164
Affiliations:
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No specific drug is currently available for the effective treatment of WNV infection. To exploit RNA interference as a potential therapeutic approach, a Moloney murine leukemia virus‐based retrovirus vector was used to effectively deliver WNV‐specific small interfering RNA (siRNA) into human neuroblastoma HTB‐11 cells. Viral plaque assays demonstrated that transduced cells were significantly refractory to WNV replication, as compared to untransduced control cells (P < 0.05), which correlated with the reduced expression of target viral genes and respective viral proteins. Therefore, retrovirus‐mediated delivery of siRNA for gene silencing can be used to study the specific functions of viral genes associated with replication and may have potential therapeutic applications. J. Med. Virol. 80:930–936, 2008. © 2008 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>Hawaï</li><li>Hubei</li></region><settlement><li>Wuhan</li></settlement><orgName><li>Université de Wuhan</li></orgName></list><tree><country name="États-Unis"><region name="Hawaï"><name sortKey="Yang, Yongbo" sort="Yang, Yongbo" uniqKey="Yang Y" first="Yongbo" last="Yang">Yongbo Yang</name></region><name sortKey="Lu, Yuanan" sort="Lu, Yuanan" uniqKey="Lu Y" first="Yuanan" last="Lu">Yuanan Lu</name><name sortKey="Lu, Yuanan" sort="Lu, Yuanan" uniqKey="Lu Y" first="Yuanan" last="Lu">Yuanan Lu</name><name sortKey="Lu, Yuanan" sort="Lu, Yuanan" uniqKey="Lu Y" first="Yuanan" last="Lu">Yuanan Lu</name><name sortKey="Nerurkar, Vivek R" sort="Nerurkar, Vivek R" uniqKey="Nerurkar V" first="Vivek R." last="Nerurkar">Vivek R. Nerurkar</name><name sortKey="Wu, Chengxiang" sort="Wu, Chengxiang" uniqKey="Wu C" first="Chengxiang" last="Wu">Chengxiang Wu</name><name sortKey="Yanagihara, Richard" sort="Yanagihara, Richard" uniqKey="Yanagihara R" first="Richard" last="Yanagihara">Richard Yanagihara</name></country><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Yang, Yongbo" sort="Yang, Yongbo" uniqKey="Yang Y" first="Yongbo" last="Yang">Yongbo Yang</name></region><name sortKey="Wu, Jianguo" sort="Wu, Jianguo" uniqKey="Wu J" first="Jianguo" last="Wu">Jianguo Wu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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